TFRC,T9,sTfR,Transferrin receptor protein 1,TR,Transferrin Receptor,P90,Transferrin Receptor (P90, CD71),CD71 Antigen,IMD46,CD71,TfR1,Trfr,TfR
Background
CD antigen CD71 is also known as Transferrin receptor protein 1, TfR, sTfR, p90, TfR1, Trfr, which belongs to the peptidase M28 family and M28B subfamily. CD71 /TFR contains one PA (protease associated) domain. CD71 / TfR1 is required for iron delivery from transferrin to cells. CD71 is a potential new target in cases of human leukomia & lymphoma. CD71 /TFRC / TfR has been shown to interact with GABARAP and HFE.
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How should species selection be planned for TfR1-targeted delivery programs?
Use human TfR1 for immunization and primary screening, then compare cynomolgus, mouse, or rat TfR1 according to the planned model. Matched construct and capture formats are essential for interpreting cross-species affinity.
Q
Which TfR1 format helps minimize avidity bias in affinity measurements?
Tag-free or His-tagged TfR1 is generally preferred when measuring monovalent kinetics. Fc-fused TfR1 can improve stability or capture but may introduce dimerization-related avidity, so results should be confirmed with a second format.
Q
When should I choose Avitag™-based or primary-amine biotinylated TfR1?
Avitag™ provides site-specific biotinylation and more controlled orientation for quantitative SPR, BLI, or ELISA. Primary-amine labeling can increase signal through multiple biotins but is more heterogeneous, so epitope accessibility should be verified.
Q
How can recombinant TfR1 and a TfR1 stable cell line be combined during shuttle or antibody screening?
Recombinant TfR1 supports controlled affinity and cross-species comparisons. A TfR1 stable cell line then tests cell-surface recognition and target engagement before transcytosis-specific models are introduced.
Q
Why can the highest-affinity TfR1 binder be a poor blood–brain barrier shuttle candidate?
TfR1-mediated transport depends on affinity, valency, and intracellular trafficking. Very tight or bivalent binding can favor receptor retention, so protein-based affinity ranking should be followed by cell transcytosis and in vivo studies.
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Human apotransferrin (Sanquin Blood Supply Foundation)
Was purchased for use in BLI, both for immobilization onto human fc binding sensors and as analyte with a biotin-labeled TfR-targeting nanobody on streptavidin.
Retested for use after 1.5 years storage at -80 and still had full functional activity.
Used this material as a captured ligand in routine SPR testing on a Biacore. Material is clean, consistent, and easy to work with, and can be used to generate quality data
We needed this product for our antibody research. ACRO managed to get it to us much faster than we expected, which made our research work very accurate. cd71 is a potential new target in human leukemia and lymphoma cases. cd71 /TFRC / TfR has been shown to interact with GABARAP and HFE. I am very pleased with the product and the quick technical support.