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Mouse VEGF164 Protein, Tag Free (HPLC-verified)

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  • 分子别名（Synonym）

    RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF

  • 表达区间及表达系统（Source）

    Mouse VEGF164 Protein, Tag Free (VE4-M4216) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 190 (Accession # AAB22253.1 ).

    Predicted N-terminus: Ala 27

    Request for sequence

  • 蛋白结构（Molecular Characterization）

    VEGF164 Structure

    This protein carries no "tag".

    The protein has a calculated MW of 19.3 kDa. The protein migrates as 23-30 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

  • 偶联（Conjugate）

    Unconjugated

  • 内毒素（Endotoxin）

    Less than 0.1 EU per μg by the LAL method.

  • 无菌（Sterility）

    Negative

  • 纯度（Purity）

    95% as determined by SDS-PAGE.

    95% as determined by SEC-HPLC.

  • 制剂（Formulation）

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法（Reconstitution）

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储（Storage）

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

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  • 电泳（SDS-PAGE）

    VEGF164 SDS-PAGE

    Mouse VEGF164 Protein, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    VEGF164 SEC-HPLC

    The purity of Mouse VEGF164 Protein, Tag Free (Cat. No. VE4-M4216) was greater than 95% as determined by SEC-HPLC.

  • 活性（Bioactivity）-ELISA

    VEGF164 ELISA

    Immobilized Mouse VEGF164 Protein, Tag Free (Cat. No. VE4-M4216) at 2 μg/mL (100 μL/well) can bind VEGFR1/R2-Fc with a linear range of 0.6-2.5 ng/mL (QC tested).

    Protocol

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背景介绍

Vascular endothelial growth factor A (VEGFA) is also known as Vascular permeability factor (VPF). VEGFA belongs to the PDGF/VEGF growth factor family. VEGFA is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. VEGFA is produced by a group of three major isoforms as a result of alternative splicing and if any three isoforms are produced (VEGFA120, VEGFA164, and VEGFA188) then this will not result in vessel defects and death of the full VEGFA knockout in mice.

前沿进展

 
药物研发进展
  • 英文全称：

    Vascular endothelial growth factor A

  • 中文全称：

    血管内皮生长因子A

  • 种类：

  • 上市药物数量：

    48 详情

  • 临床药物数量：

    65 详情

  • 最高研发阶段：

    批准上市

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