产品详情
分子别名(Synonym)
TSLP
表达区间及表达系统(Source)
Cynomolgus TSLP, His Tag (TSP-C52H8) is expressed from human 293 cells (HEK293). It contains AA Tyr 29 - Gln 159 (Accession # A0A7N9CAT7-1).
Predicted N-terminus: Tyr 29
Request for sequence蛋白结构(Molecular Characterization)
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 17.1 kDa. The protein migrates as 8-10 kDa, 15-20 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联(Conjugate)
Unconjugated
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
90% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系(QMS)
产品展示
电泳(SDS-PAGE)
Cynomolgus TSLP, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性(Bioactivity)-ELISA
Immobilized Cynomolgus TSLP, His Tag (Cat. No. TSP-C52H8) at 5 μg/mL (100 μL/well) can bind Human TSLP R, Fc Tag (Cat. No. TSR-H525a) with a linear range of 2-31 ng/mL (QC tested).
ProtocolImmobilized Cynomolgus TSLP, His Tag (Cat. No. TSP-C52H8) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human TSLP Antibody, Human IgG2 with a linear range of 1-16 ng/mL (Routinely tested).
Protocol
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