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Cynomolgus PSMA / FOLH1 Protein, His Tag (active enzyme, MALS verified)

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  • 分子别名（Synonym）

    FOLH1,PSMA,GIG27,FOLH,NAALAD1,PSM,NAALADase I,GCPII,FGCP

  • 表达区间及表达系统（Source）

    Cynomolgus PSMA, His Tag (PSA-C5247) is expressed from human 293 cells (HEK293). It contains AA Lys 44 - Ala 750 (Accession # A0A2K5VNZ0-1).

    Predicted N-terminus: His

    Request for sequence

  • 蛋白结构（Molecular Characterization）

    PSMA Structure

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 81.4 kDa. The protein migrates as 100-115 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 偶联（Conjugate）

    Unconjugated

  • 内毒素（Endotoxin）

    Less than 1.0 EU per μg by the LAL method.

  • 纯度（Purity）

    95% as determined by SDS-PAGE.

  • 制剂（Formulation）

    Supplied as 0.2 μm filtered solution in MES and NaCl with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 运输（Shipping）

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • 存储（Storage）

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

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  • 电泳（SDS-PAGE）

    PSMA SDS-PAGE

    Cynomolgus PSMA, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    PSMA SEC-MALS

    The purity of Cynomolgus PSMA, His Tag (Cat. No. PSA-C5247) is more than 85% and the molecular weight of this protein is around 185-220 kDa verified by SEC-MALS.

    Report

  • 活性（Bioactivity）-ELISA

    PSMA ELISA

    Immobilized Cynomolgus PSMA, His Tag (Cat. No. PSA-C5247) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human PSMA Antibody, Human IgG1 with a linear range of 0.2-4 ng/mL (QC tested).

    Protocol

  • 活性（Bioactivity）

    Measured by its ability to hydrolyze the substrate N-acetyl-L-Asp-L-Glu into N-acetyl-L-Asp and L-Glu. The L-Glu product is measured by fluorescence after its derivatization by ortho-phthaldialdehyde. The specific activity is >250 pmol/min/μg, as measured under the described conditions (QC tested).

    Protocol

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背景介绍

Prostate-specific membrane antigen (PSMA) is also known as Folate hydrolase 1 (FOLH1), Glutamate carboxypeptidase 2 (GCP2), N-acetylated-alpha-linked acidic dipeptidase I (NAALAD1), which belongs to the peptidase M28 family and M28B subfamily. FOLH1 / PSMA is stable at pH greater than 6.5. FOLH1 / PSMA is a type II transmembrane zinc metallopeptidase that is most highly expressed in the nervous system, prostate, kidney, and small intestine. FOLH1 / GCP-2 is homodimer and binds 2 zinc ions per subunit, and required for NAALADase activity. The catalytic activity of PSMA involved in releasing of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly – gamma - glutamates. FOLH1 / GCP-2 / PSMA has both folate hydrolase and N – acetylated – alpha – linked - acidic dipeptidase (NAALADase) activity and has a preference for tri-alpha-glutamate peptides. GCP-2 / PSMA involved in prostate tumor progression and also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.

前沿进展

 
药物研发进展
  • 英文全称：

    Prostate-Specific Membrane Antigen

  • 中文全称：

    前列腺癌特异性膜抗原

  • 种类：

  • 上市药物数量：

    5 详情

  • 临床药物数量：

    102 详情

  • 最高研发阶段：

    临床三期

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