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Human NFL Protein, His Tag

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  • 分子别名(Synonym)

    Neurofilament light polypeptide

  • 表达区间及表达系统(Source)

    Human NFL, His Tag (NFL-H5143) is expressed from E. coli cells. It contains AA Ser 2 - Asp 543 (Accession # P07196-1).

    Predicted N-terminus: Met

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    NFL Structure

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 63.4 kDa.

  • 偶联(Conjugate)

    Unconjugated

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method.

  • 纯度(Purity)

    90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Supplied as 0.2 μm filtered solution in PBS, pH7.4 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • 运输(Shipping)

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • 存储(Storage)

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 12 months under sterile conditions.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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  • 活性(Bioactivity)-ELISA

     NFL ELISA

    Immobilized Human NFL, His Tag (Cat. No. NFL-H5143) at 1 μg/mL (100 μL/well) can bind Anti NEFL Antibody with a linear range of 1-16 ng/mL (QC tested).

    Protocol

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背景介绍

Neurofilaments (NF) are classed as type IV intermediate filaments found in the cytoplasm of neurons. Together with microtubules and microfilaments, they form the neuronal cytoskeleton. Neurofilaments usually contain three intermediate filament proteins: NFL, NFM, and NFH which are involved in the maintenance of neuronal caliber. Neurofilament heavy (NFH) can server as biomarkers for several diseases by indicating axonal injury.

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