选择语言
免费注册积分兑换
关注公众号
我的购物车
收藏产品

Influenza A [Guangdong/18SF020(H5N6)] Neuraminidase (NA) Protein, His Tag (MALS & SPR verified)

COAMS
DS
用户评价
货号-规格
价格
Qty.
合计0件 产品金额0
0
加入购物车
新品建议
增项建议

产品详情

  • 分子别名（Synonym）

    Neuraminidase/NA (Influenza Virus)

  • 表达区间及表达系统（Source）

    Influenza A [Guangdong/18SF020(H5N6)] Neuraminidase (NA) Protein, His Tag (NEE-V524h) is expressed from human 293 cells (HEK293). It contains AA Leu 35 - Lys 459 (Accession # EPI1366590, GISAID).

    Predicted N-terminus: His

    Request for sequence

  • 蛋白结构（Molecular Characterization）

    Neuraminidase/NA (Influenza Virus) Structure

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 55.2 kDa. The protein migrates as 65-75 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 偶联（Conjugate）

    Unconjugated

  • 内毒素（Endotoxin）

    Less than 1.0 EU per μg by the LAL method.

  • 纯度（Purity）

    90% as determined by SDS-PAGE.

  • 制剂（Formulation）

    Supplied as 0.2 μm filtered solution in PBS, 300 mM NaCl, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 运输（Shipping）

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • 存储（Storage）

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

产品展示

  • 电泳（SDS-PAGE）

    Neuraminidase/NA (Influenza Virus) SDS-PAGE

    Influenza A [Guangdong/18SF020(H5N6)] Neuraminidase (NA) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Neuraminidase/NA (Influenza Virus) SEC-MALS

    The purity of Influenza A [Guangdong/18SF020(H5N6)] Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V524h) is more than 85% and the molecular weight of this protein is around 270-300 kDa verified by SEC-MALS.

    Report

  • 活性（Bioactivity）-SPR

    Neuraminidase/NA (Influenza Virus) SPR

    a-Neu5Ac-PAA-biotin immobilized on SA Chip can bind Influenza A [Guangdong/18SF020(H5N6)] Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V524h) with an affinity constant of 42.7 nM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol

  • Neuraminidase/NA (Influenza Virus) SPR

    Influenza A [Guangdong/18SF020(H5N6)] Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V524h) immobilized on CM5 Chip can bind N-Acetylneuraminic Acid with an affinity constant of 128 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

用户评价
发表评论

背景介绍

Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme which facilitates virus release and efficient spread of the progeny virus from cell to cell.

前沿进展

 
联系我们
项目合作
查看更多项目
  • 产品SKU
  • 产品详情
  • 产品展示
  • 用户评价
  • 背景介绍