产品详情
分子别名(Synonym)
MMP2,CLG4,CLG4A,MMP-II,MONA,TBE-1
表达区间及表达系统(Source)
Mouse MMP-2 (30-460), His Tag (MM2-M52H9) is expressed from human 293 cells (HEK293). It contains AA Ala 30 - Thr 460 (Accession # P33434-1). It needs to be activated by agents such as APMA in vitro to have hydrolytic activity.
Predicted N-terminus: Ala 30
Request for sequence蛋白结构(Molecular Characterization)
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 50.0 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联(Conjugate)
Unconjugated
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
85% as determined by SDS-PAGE.
制剂(Formulation)
Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, 20% glycerol, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
运输(Shipping)
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储(Storage)
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 6 months under sterile conditions.
质量管理控制体系(QMS)
产品展示
电泳(SDS-PAGE)
Mouse MMP-2 (30-460), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 85%.
活性(Bioactivity)
Measured by its ability to cleave the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2. The specific activity is >1,500 pmol/min/µg (QC tested).
Protocol
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