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分子别名（Synonym）
IL7Ra,CD127
表达区间及表达系统（Source）
Biotinylated Human IL-7 R alpha Protein, Fc,Avitag (IL7-H82F9) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Asp 239 (Accession # P16871-1).
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 53.5 kDa. the protein migrates as 67-75 kDa under reducing (R) condition, and >116 kDa under non-reducing (NR) condition (SDS-PAGE).
偶联（Conjugate）
Biotin
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Biotinylated Human IL-7 R alpha Protein, Fc,Avitag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%undefined.
活性（Bioactivity）-ELISA
Immobilized Human IL-7, Fc Tag (Cat. No. IL7-H5253) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-7 R alpha Protein, Fc,Avitag (Cat. No. IL7-H82F9) with a linear range of 1-20 ng/mL (QC tested).
Protocol
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