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分子别名（Synonym）
IL6R,CD126,IL-6R-1,IL-6RA,IL6RA,IL-6R-alpha,IL6RQ,gp80
表达区间及表达系统（Source）
Biotinylated Human IL-6 R alpha, Avitag,His Tag (CD6-H82E8) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 365 (Accession # P08887-1).
Predicted N-terminus: Leu 20
Request for sequence蛋白结构（Molecular Characterization）
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 41.7 kDa. The protein migrates as 52-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Biotin
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Biotinylated Human IL-6 R alpha, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human IL-6 R alpha, Avitag,His Tag (Cat. No. CD6-H82E8) is more than 85% and the molecular weight of this protein is around 65-80 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human IL-6, premium grade (Cat. No. IL6-H4218) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-6 R alpha, Avitag,His Tag (Cat. No. CD6-H82E8) with a linear range of 4-63 ng/mL (QC tested).
Protocol
活性（Bioactivity）-BLI
Loaded Biotinylated Human IL-6 R alpha, Avitag,His Tag (Cat. No. CD6-H82E8) on SA Biosensor, can bind Human IL-6, premium grade (Cat. No. IL6-H4218) with an affinity constant of 73.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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