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Cynomolgus IL-31 Protein, His Tag

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  • 分子别名(Synonym)

    IL-31,IL31,Interleukin-31

  • 表达区间及表达系统(Source)

    Cynomolgus IL-31, His Tag (IL1-C5245) is expressed from human 293 cells (HEK293). It contains AA Leu 27 - Thr 163 (Accession # G7PJ78-1).

    Predicted N-terminus: His

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    IL-31 Structure

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 17.3 kDa. The protein migrates as 20-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 偶联(Conjugate)

    Unconjugated

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method.

  • 纯度(Purity)

    90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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  • 电泳(SDS-PAGE)

    IL-31 SDS-PAGE

    Cynomolgus IL-31, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • 活性(Bioactivity)-ELISA

     IL-31 ELISA

    Immobilized Cynomolgus IL-31, His Tag (Cat. No. IL1-C5245) at 1 μg/mL (100 μL/well) can bind Cynomolgus/Rhesus macaque IL-31 RA Protein, Fc Tag (Cat. No. ILA-H525b) with a linear range of 5-78 ng/mL (QC tested).

    Protocol

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背景介绍

IL-31 is an inflammatory cytokine that helps trigger cell-mediated immunity against pathogens. Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. It has also been identified as a major player in a number of chronic inflammatory diseases, including atopic dermatitis. May function in skin immunity. IL-31 is produced by a variety of cells, namely type 2 helper (TH2) T-cells. IL-31 sends signals through a receptor complex made of IL-31RA and oncostatin M receptor β (OSMRβ) expressed in immune and epithelial cells. These signals activate three pathways: ERK1/2 MAP kinase, PI3K/AKT, and JAK1/2 signaling pathways.

前沿进展

 
药物研发进展
  • 英文全称:

    Interleukin-31

  • 中文全称:

    白介素31

  • 种类:

  • 上市药物数量:

    0 详情

  • 临床药物数量:

    2 详情

  • 最高研发阶段:

    临床一期

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