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分子别名（Synonym）
IL-15,Interleukin-15,MGC9721
表达区间及表达系统（Source）
Human IL-15, premium grade (IL5-H4117) is expressed from E. coli cells. It contains AA Asn 49 - Ser 162 (Accession # P40933-1).
Predicted N-terminus: Met
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage.
GMP-L15H13 is the GMP version of this IL5-H4117. These two proteins display indistinguishable performance profiles, thereby ensuring a seamless transition for end users from early preclinical stag to later clinical phases.Request for sequence蛋白结构（Molecular Characterization）
This protein carries no "tag".
The protein has a calculated MW of 12.9 kDa. The protein migrates as 13 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 0.01 EU per μg by the LAL method.
宿主蛋白残留（Host Cell Protein）
0.5 ng/µg of protein tested by ELISA.
宿主核酸残留（Host Cell DNA）
0.02 ng/μg of protein tested by qPCR.
无菌（Sterility）
Negative
支原体（Mycoplasma）
Negative.
纯度（Purity）
95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 25 mM Histidine, pH6.2 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Human IL-15, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-CELL BASE
Human IL-15, premium grade (Cat. No. IL5-H4117) stimulates proliferation of CTLL-2 cells. The specific activity of Human IL-15, premium grade is > 8.00ⅹ10^6 IU/mg, which is calibrated against human IL-15 WHO International Standard (NIBSC code: 95/554) (QC tested).
Protocol
Human IL-15, premium grade (Cat. No. IL5-H4117), designed for preclinical stage, has the same activity and performance with GMP Human IL-15 (Cat. No. GMP-L15H13), which enables a seamless transition from preclinical development to clinical phases.
Protocol
活性（Bioactivity）-ELISA
Immobilized Human IL-15, premium grade (Cat. No. IL5-H4117) at 2 μg/mL (100 μL/well) can bind Human IL-15 R alpha, Fc Tag (Cat. No. ILA-H5253) with a linear range of 0.6-39 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Human IL-2 R beta, Fc Tag (Cat. No. ILB-H5253) captured on CM5 chip via anti-human IgG Fc antibody, can bind Human IL-15, premium grade (Cat. No. IL5-H4117) with an affinity constant of 9.5 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Human IL-2 R beta, Fc Tag (Cat. No. ILB-H5253) on Protein A Biosensor, can bind Human IL-15, premium grade (Cat. No. IL5-H4117) with an affinity constant of 10.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
稳定性（Stability）
The Cell based assay shows batch-to-batch consistency between Acro's GMP and PG IL-15.
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