产品详情
分子别名(Synonym)
Hemagglutinin/HA (Influenza Virus)
表达区间及表达系统(Source)
Influenza B [Phuket/3073/2013 (B/Yamagata lineage)] HA Protein, His Tag (HAE-V52H4) is expressed from human 293 cells (HEK293). It contains AA Asp 16- Ser 558 (Accession # EPI529345, GISAID).
Predicted N-terminus: Asp 16
Request for sequence蛋白结构(Molecular Characterization)
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 64.1 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联(Conjugate)
Unconjugated
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
90% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, pH5.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系(QMS)
产品展示
电泳(SDS-PAGE)
Influenza B [Phuket/3073/2013 (B/Yamagata lineage)] HA Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
活性(Bioactivity)-ELISA
Immobilized Influenza B [Phuket/3073/2013 (B/Yamagata lineage)] HA Protein, His Tag (Cat. No. HAE-V52H4) at 1 μg/mL (100 μL/well) can bind Anti-HA (B/Phuket/3073/2013) Monoclonal Antibody, Mouse IgG with a linear range of 0.5-63 ng/mL (QC tested).
Protocol
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