产品详情
分子别名(Synonym)
Hemagglutinin 1/HA 1 (Influenza Virus)
表达区间及表达系统(Source)
Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] HA1 Protein, His Tag (HA1-V52H5) is expressed from human 293 cells (HEK293). It contains AA Asp 19 - Arg 338 (Accession # Q80KD9_9INFA).
Predicted N-terminus: Asp 19
Request for sequence蛋白结构(Molecular Characterization)
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 40.9 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联(Conjugate)
Unconjugated
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
90% as determined by SDS-PAGE.
90% as determined by SEC-MALS.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, pH5.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系(QMS)
产品展示
电泳(SDS-PAGE)
Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] HA1 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] HA1 Protein, His Tag (Cat. No. HA1-V52H5) is more than 90% and the molecular weight of this protein is around 170-190 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA
Immobilized Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] Hemagglutinin 1 (HA1) Protein, His Tag (Cat. No. HA1-V52H5) at 1 μg/mL (100 μL/well) can bind Anti-HA Monoclonal Antibody (H9N2) with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
用户评价 发表评论
