积分兑换
关注公众号
收藏产品 产品详情
分子别名（Synonym）
Glycoprotein B/gB (HCMV)
表达区间及表达系统（Source）
HCMV Glycoprotein B (gB) Protein (Strain AD169), His Tag is the ectodomain of HCMV (strain AD169) glycoprotein B (gB) which contains AA Ser 25 - Phe 706 (Accession # P06473 (Y155G, I156H, Y157R, Y206H, W240A, L241T, Y242H, R456S, R459S)). The recombinant protein is expressed from human 293 cells (HEK293) with a polyhistidine tag at the C-terminus. Mutations Y155G, I156H, Y157R, Y206H, W240A, L241T, Y242H and R456S, R459S are introduced to stabilize the trimeric postfusion state of HCMV gB protein and abolish the furin cleavage site, respectively.
Predicted N-terminus: Ser 25
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 79.6 kDa. The protein migrates as 90-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
95% as determined by SDS-PAGE.
95% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
HCMV (strain AD169) Glycoprotein B (gB), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of HCMV (strain AD169) Glycoprotein B (gB), His Tag (Cat. No. CMB-V52H4) is more than 95% and the molecular weight of this protein is around 308-376 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized HCMV (strain AD169) Glycoprotein B (gB), His Tag (Cat. No. CMB-V52H4) at 1 μg/mL (100 μL/well) can bind Anti-gB(HCMV)-antibody-SM5-1,Human IgG1(AM330) with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
Immobilized Anti-gB(HCMV)-antibody-SM5-1,Human IgG1(AM330) at 1 μg/mL (100 μL/well) can bind HCMV (strain AD169) Glycoprotein B (gB), His Tag (Cat. No. CMB-V52H4) with a linear range of 0.1-8 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Anti-glycoprotein B antibody-SM5-1 captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind HCMV (strain AD169) Glycoprotein B (gB), His Tag (Cat. No. CMB-V52H4) with an affinity constant of 5.59 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
用户评价 发表评论
联系我们 
项目合作