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Epstein-Barr virus Envelope Glycoprotein B (gB), His Tag (MALS verified)

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  • 分子别名(Synonym)

    Glycoprotein B/gB (EBV)

  • 表达区间及表达系统(Source)

    Epstein-Barr virus Envelope Glycoprotein B (gB), His Tag (GLB-E52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Gly 685 (Accession # P03188 (W112H, Y113R, R428G, R429S, R430G, R431S, R432G, W193R, L194V, I195E, W196A)).

    Predicted N-terminus: Gln 23

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 76.0 kDa. The protein migrates as 90-100 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 偶联(Conjugate)

    Unconjugated

  • 内毒素(Endotoxin)

    Less than 0.9 EU per μg by the LAL method.

  • 纯度(Purity)

    95% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, 0.1 M Arginine, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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  • 电泳(SDS-PAGE)

    Glycoprotein B/gB (EBV) SDS-PAGE

    Epstein-Barr virus Envelope Glycoprotein B (gB), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Glycoprotein B/gB (EBV) SEC-MALS

    The purity of Epstein-Barr virus Envelope Glycoprotein B (gB), His Tag (Cat. No. GLB-E52H3) is more than 85% and the molecular weight of this protein is around 275-305 kDa verified by SEC-MALS.

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背景介绍

Epstein-Bar Virus (EBV), also known as human herpesvirus 4, belongs to gamma herpes virus family and is a very common human virus worldwide. EBV causes infectious mononucleosis (IM) and also associates to some specific types of cancers such as Burkitt’s lymphoma (BL) and gastric carcinoma (GC). Glycoprotein B (gB) plays an important role in viral entry by binding with αvβ6/αvβ8 integrins to trigger the membrane fusion and entry process of epithelial cells, which makes it become an great target for EBV research.

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