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Human FGF-7 / HBGF-7 / KGF Protein, His Tag (HPLC & SPR verified)

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  • 分子别名（Synonym）

    FGF-7,Fibroblast growth factor 7,HBGF-7,Keratinocyte growth factor,KGF

  • 表达区间及表达系统（Source）

    Human FGF-7 Protein, His Tag (FG7-H52H5) is expressed from human 293 cells (HEK293). It contains AA Cys 32 - Thr 194 (Accession # P21781-1).

    Request for sequence

  • 蛋白结构（Molecular Characterization）

    FGF-7 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 20.9 kDa. The protein migrates as 25-27 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • 偶联（Conjugate）

    Unconjugated

  • 内毒素（Endotoxin）

    Less than 0.1 EU per μg by the LAL method.

  • 无菌（Sterility）

    Negative

  • 支原体（Mycoplasma）

    Negative.

  • 纯度（Purity）

    95% as determined by SDS-PAGE.

    95% as determined by SEC-HPLC.

  • 制剂（Formulation）

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法（Reconstitution）

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储（Storage）

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

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  • 电泳（SDS-PAGE）

    FGF-7 SDS-PAGE

    Human FGF-7 Protein, His Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    FGF-7 SEC-HPLC

    The purity of Human FGF-7 Protein, His Tag (Cat. No. FG7-H52H5) was greater than 95% as determined by SEC-HPLC.

  • 活性（Bioactivity）-Organoid Culture

    FGF-7 ORGANOID CULTURE

    Human EGF (Cat. No. EGF-H52H3), Noggin (Cat. No. NON-H5257), R-spondin1 (Cat. No. RS6-H4220), FGF7 (Cat. No. FG7-H52H5), FGF10, HGF (Cat. No. HGF-H52H3) actively support liver ductal organoid growth.

  • 活性（Bioactivity）-SPR

    FGF-7 SPR

    Human FGF R2 (IIIb), Fc Tag (Cat. No. FGB-H5256) immobilized on CM5 Chip can bind Human FGF-7 Protein, His Tag (Cat. No. FG7-H52H5) with an affinity constant of 0.275 μM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol

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背景介绍

Fibroblast growth factor (FGF) 7 (is also known as Keratinocyte growth factor (KGF)), a member of FGF family, is initially found to be secreted from mesenchymal cells to repair epithelial tissues. As a well-characterized paracrine growth factor for tissue growth and regeneration, fibroblast growth factor 7 (FGF7) is involved in a number of physiological and pathological processes, including lung disease and cancer. The stromal-derived FGFs, such as FGF7 and FGF10, control epithelial cell resident FGFR2IIIb activities, promote net tissue homeostasis, and restraint tumor cells from progression to malignancy.

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