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分子别名（Synonym）
FcRn,FCGRT & B2M
表达区间及表达系统（Source）
Mouse FCGRT&B2M Heterodimer Protein, His Tag (FCM-M52W8) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # Q61559-1 (FCGRT) & P01887-1 (B2M)).
Predicted N-terminus: Ser 22 (FCGRT) & Ile 21 (B2M)
Request for sequence蛋白结构（Molecular Characterization）
Mouse FCGRT&B2M Heterodimer Protein, His Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 32.9 kDa (FCGRT) and 11.6 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) contains no tag at the C-terminus. The reducing (R) protein migrates as 45-50 kDa (FCGRT) and 10 kDa (B2M) respectively due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
95% as determined by SDS-PAGE.
95% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Mouse FCGRT&B2M Heterodimer Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%undefined.
SEC-MALS
The purity of Mouse FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCM-M52W8) is more than 95% and the molecular weight of this protein is around 45-60 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-SPR
Mouse FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCM-M52W8) captured on NTA-Series S sensor chip can bind Herceptin with an affinity constant of 2.52 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Biotinylated Mouse Serum Albumin, His,Avitag (Cat. No. MSA-M82E4) immobilized on SA Chip can bind Mouse FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCM-M52W8) with an affinity constant of 377 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Mouse Serum Albumin, His Tag (Cat. No. MSA-M52H8) immobilized on CM5 Chip can bind Mouse FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCM-M52W8) with an affinity constant of 0.376 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Mouse FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCM-M52W8) on NTA Biosensor, can bind Herceptin with an affinity constant of 1.13 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
Loaded Mouse FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCM-M52W8) on NTA Biosensor, can bind Mouse IgG Fc, Tag Free (Cat. No. IG1-M5208) with an affinity constant of 14.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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