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分子别名（Synonym）
CD73,NT5E,5'-Nucleotidase,5'-NT,NT5,NTE
表达区间及表达系统（Source）
Human CD73 Protein, His Tag (CD3-H52H7) is expressed from human 293 cells (HEK293). It contains AA Trp 27 - Ser 549 (Accession # P21589-1).
Predicted N-terminus: Trp 27
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 59.9 kDa. The protein migrates as 65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
90% as determined by SDS-PAGE.
90% as determined by SEC-HPLC.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 120 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Human CD73 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human CD73 Protein, His Tag (Cat. No. CD3-H52H7) was greater than 90% as determined by SEC-HPLC.
活性（Bioactivity）-ELISA
Immobilized Human CD73 Protein, His Tag (Cat. No. CD3-H52H7) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD73 Antibody, Human IgG1 with a linear range of 0.15-5 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Anti-CD73 antibody (Human IgG1) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human CD73 Protein, His Tag (Cat. No. CD3-H52H7) with an affinity constant of 0.164 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Anti-CD73 Antibody captured on Protein A Chip can bind Human CD73 Protein, His Tag (Cat. No. CD3-H52H7) with an affinity constant of 0.108 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Anti-CD73 MAb (Human IgG1) on AHC Biosensor, can bind Human CD73 Protein, His Tag (Cat. No. CD3-H52H7) with an affinity constant of 0.179 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
活性（Bioactivity）
Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The specific activity is >100000pmol/min/μg (QC tested).
Protocol
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