产品详情
分子别名(Synonym)
BG, MPS7
表达区间及表达系统(Source)
Human beta-Glucuronidase Protein, His Tag (BEB-H52H3) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Thr 651 (Accession # P08236).
Predicted N-terminus: Leu 23
Request for sequence蛋白结构(Molecular Characterization)
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 74.5 kDa. The protein migrates as 75-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联(Conjugate)
Unconjugated
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
95% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in 50 mM Tris,150 mM NaCI,pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系(QMS)
产品展示
电泳(SDS-PAGE)
Human beta-Glucuronidase Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性(Bioactivity)
Measured by its ability to hydrolyze 4-methylumbelliferyl-beta -D-glucuronide. The specific activity is >3500 pmol/min/μg(QC tested).
Protocol
用户评价 发表评论
