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分子别名（Synonym）
TNFRSF17,CD269,BCM,BCMA
表达区间及表达系统（Source）
Human BCMA, His Tag, premium grade (BCA-H522y) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 54 (Accession # Q02223-1).
Predicted N-terminus: Met 1
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 7.8 kDa. The protein migrates as 12 kDa and 14-18 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 0.01 EU per μg by the LAL method.
无菌（Sterility）
Negative
支原体（Mycoplasma）
Negative.
纯度（Purity）
90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Human BCMA, His Tag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human BCMA, His Tag, premium grade (Cat. No. BCA-H522y) was greater than 80% as determined by SEC-HPLC.
活性（Bioactivity）-FACS
2e5 of BCMA-CAR-293 cells transfected with anti-BCMA-scFv were stained with 100 μL of 0.3 μg/mL of Human BCMA, His Tag, premium grade (Cat. No. BCA-H522y) and negative control protein respectively, washed and then followed by FITC anti-6xHis tag antibody and analyzed with FACS (Routinely tested).
Protocol
活性（Bioactivity）-ELISA
Immobilized Human BAFF, Fc Tag at 5 μg/mL (100 μL/well) can bind Human BCMA, His Tag, premium grade (Cat. No. BCA-H522y) with a linear range of 0.031-0.5 μg/mL (QC tested).
Protocol
Immobilized Human APRIL, Fc Tag at 5 μg/mL (100 μL/well) can bind Human BCMA, His Tag, premium grade (Cat. No. BCA-H522y) with a linear range of 6-25 ng/mL (Routinely tested).
Protocol
Immobilized Human BCMA, His Tag, premium grade (Cat. No. BCA-H522y) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human BCMA Antibody, Human IgG1 with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
Immobilized Human BCMA, His Tag, premium grade (Cat. No. BCA-H522y) at 2 μg/mL (100 μL/well) can bind Anti-BCMA Neutralizing Antibody, Mouse IgG1 with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Anti-Human BCMA MAb (human IgG1) captured on CM5 chip via Anti-Human IgG Fc antibodies surface, can bind Human BCMA, His Tag, premium grade (Cat. No. BCA-H522y) with an affinity constant of 13.0 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Anti-BCMA Neutralizing Antibody, Mouse IgG1 captured on CM5 chip via anti-mouse antibodies surface can bind Human BCMA, His Tag, premium grade (Cat. No. BCA-H522y) with an affinity constant of 0.234 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Anti-Human BCMA MAb (human IgG1) on Protein A Biosensor, can bind Human BCMA, His Tag, premium grade (Cat. No. BCA-H522y) with an affinity constant of 29.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human APRIL, Fc Tag on Protein A Biosensor, can bind Human BCMA, His Tag, premium grade (Cat. No. BCA-H522y) with an affinity constant of 10.9 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human BAFF, Fc Tag on Protein A Biosensor, can bind Human BCMA, His Tag, premium grade (Cat. No. BCA-H522y) with an affinity constant of 0.64 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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