产品详情
组分(Materials Provided)
IDComponentsSizeRAS197-C01Pre-coated HSV-2 (strain 333) Envelope Glycoprotein C (gC) Microplate1 plateRAS197-C02HSV-2 (strain 333) (gC) Antibody Positive Control100 μLRAS197-C03HSV-2 (strain 333) (gC) Antibody Negative Control100 μLRAS197-C04HRP-Conjugated Antibody50 μLRAS197-C0510×Washing Buffer50 mLRAS197-C06Dilution Buffer50 mLRAS197-C07Substrate Solution12 mLRAS197-C08Stop Solution7 mL产品概述(Product Overview)
Herpesvirus infections are widely spread throughout the world population. Herpes simplex virus (HSV) belongs to the α-herpesvirus subfamily. There are two main types of HSV, HSV-1 and HSV-2, which infect humans. HSV-2 mainly causes genital lesions, whereas HSV-1 is involved in both oral and genital infections. Glycoprotein C (gC) is a structural component of the herpes simplex virus type 2 (HSV-2) envelope that mediates binding of the virus to cell surface heparan sulfate or chondroitin sulfate. Also plays a role in host immune evasion by inhibiting the host complement cascade activation (By similarity).
应用说明(Application)
The kit is developed for titer measurement of Anti-HSV-2 Envelope Glycoprotein C (gC) antibody IgG in mouse serum.
It is for research use only.
存储(Storage)
2-8℃
原理(Assay Principles)
This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-HSV-2 (strain 333) (gC) antibodies in Mouse serum by HSV-2 (strain 333) Envelope Glycoprotein C (gC). The Kit consists of Pre-coated HSV-2 (strain 333) Envelope Glycoprotein C (gC) Microplate,Positive Control,Negative Control and HRP-conjugated antibody.
Your experiment will include 4 simple steps:
a) The samples and Control are diluted by Dilution Buffer.Add your sample to the plate.
b) Add the HRP-conjugated antibody diluted by Dilution Buffer to the plate.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
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