resDetect™ Cas9 (CRISPR Associated Protein 9) ELISA Kit (Residue Testing)

产品参数（Product Specifications）

产品概述（Product Overview）

resDetect™ Cas9 (CRISPR Associated Protein 9) ELISA Kit (Residue Testing) is based on the Sandwich-ELISA method and is used for the quantitative detection of Cas9 residues in cell therapy products. It is designed to provide a reliable tool for supporting quality control in gene and cell therapy manufacturing by monitoring Cas9 residues during in-process testing and final product release, ensuring safety, and compliance in CRISPR-based therapeutic applications.

应用说明（Application）

resDetect™ Cas9 (CRISPR Associated Protein 9) ELISA Kit (Residue Testing) is developed for quantitative detection of Cas9 in cell Therapy.

It is for research use only.

重构方法（Reconstitution）

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储（Storage）

2-8℃

组分（Materials Provided）

原理（Assay Principles）

This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Cas9 Nuclease. The kit consists of Pre-coated Anti-Cas9 Antibody Microplate and Cas9 Nuclease Standard and Biotin-Anti-Cas9 Antibody and Streptavidin-HRP and buffers.

Your experiment will include 6 simple steps:

a) Bring all reagents to room temperature(20℃-25℃) before use.

b) Add your sample to the plate and take the Cas9 Nuclease Standard. The samples and standard are diluted by Dilution Buffer.

c) Wash the plate and add the Biotin-Anti-Cas9 Antibody diluted by Dilution Buffer to the plate.

d) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.

e) Wash the plate and add TMB.

f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound protein.

质量管理控制体系（QMS）

1. 质量管理体系（ISO, GMP）
2. 质量优势
3. 质控流程