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HEK293/Human Claudin-1 Stable Cell Line

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    1. Genetically modified cell lines best reflect MOA (Mechanism of Action)
    2. Higher activity and larger assay window for robust and reproducible cell-based bioassay
    3. Comprehensive application data to support assay development and validation
    4. Full tracible record, stringent quality control and validated cell passage stability
    5. Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
    6. Global commercial license assistance whenever regulatory filing is required

  • 描述（Description）

    The HEK293/Human Claudin-1 Stable Cell Line was engineered to express the receptor full length human Claudin-1 (Gene ID: 9076), used to mimic cancer target cells. Surface expression of Human Claudin-1 was confirmed by flow cytometry.

  • 应用说明（Application）

    • Useful for cell-based Claudin-1 binding assay

  • 生长特性（Growth Properties）

    Adherent

  • 筛选标记（Selection Marker）

    Puromycin (2 μg/mL)

  • 培养基（Complete Growth Medium）

    DMEM + 10% FBS

  • 冻存液（Freeze Medium）

    Serum-free cell cryopreservation medium

  • 装量（Quantity）

    1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

  • 存储（Storage）

    Frozen in liquid nitrogen.

  • 支原体检测（Mycoplasma Testing）

    Negative

  • 无菌检测（Sterility Testing）

    Negative

  • 使用说明（Instructions for Use）

    See data sheet for detailed culturing and assay protocol.

产品展示

  • Receptor Assay

    Claudin-1 FACS

    Expression analysis of human Claudin-1 on HEK293/Human Claudin-1 Stable Cell Line by FACS.
    Cell surface staining was performed on HEK293/Human Claudin-1 Stable Cell Line or negative control cell using
    anti-human Claudin-1 antibody followed by staining with FITC anti-mouse IgG Fc Antibody.

    Protocol

  • Passage Stability

    Claudin-1 PASSAGE

    Passage stability analysis of receptors expression by FACS.
    Flow cytometry surface staining of human Claudin-1 on HEK293/Human Claudin-1 Stable Cell Line demonstrates consistent mean fluorescent intensity across across passage 6-21.

    Protocol
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背景介绍

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. [provided by RefSeq, Jul 2008]

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