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- ItemsDetailsDetection MethodChemiluminescenceProduct TypeMagnetic Beads (Anti-Mouse IgG)Quantity Size10mg / 100mgPhysical Appearancelyophilized powder mixtureParticle size2.8 µmBeads SurfaceHydrophilicAmount of Coupled ProteinAbout 133 pmol (20 μg) Monoclonal Rabbit anti-mouse IgG antibody / mg BeadsBinding Capacity2-5ug mouse antibody or Mouse IgG Fc tag proteins / mg beadsEmission WavelengthMeasured relative light units (RLU) at 430 nm
背景（Background）
The Anti-Mouse IgG-coupled Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly affinity Monoclonal Rabbit anti-mouse IgG antibody. The beads can be used to capture the mouse IgG in Chemiluminescence procedures. The antibody reacts with the heavy and light chain of mouse IgG. It can recognizes mouse IgG1, IgG2a, IgG2b and it binds IgG3 with a lower reactivity. It recognizes rat IgG and has no cross-reactivity with chicken IgY, goat IgG, human IgG, and rabbit IgG. The Anti-Mouse IgG-coupled Magnetic Beads is easy to capture the mouse IgG, and the bounded protein have no activity lost, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.
应用说明（Application）
The Anti-Mouse IgG-coupled Magnetic Beads is used to capture the mouse IgG, it can combination with Acridinium ester markers in chemiluminescence technology, The Acridinium ester markers such as Streptavidin-Acridinium ester can capture the biotinylated proteins or molecules, this allows detection of antigen and antibody binding or antibody screening.
* The Isotype control (Cat. No. SMB-B01) is sold separately.
重构方法（Reconstitution）
See Certificate of Analysis (CoA) for detailed instruction.
存储（Storage）
Upon receipt, please store the Beads at -20°C. The shelf life is 1 year at -20 °C.
Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.
制剂（Formulation）
Please contact us for detailed information.
Contact us for customized product form or formulation.
质量管理控制体系（QMS）
产品展示
活性（Bioactivity）-MPCLIA
Immobilized 0.04 μg /Test of Biotinylated Human PD-1, Avitag,His Tag (Cat. No. PD1-H82E4) to the Streptavidin-Acridine ester (Cat. No. STN-NA114, 0.008 μg /Test), incubated with 100 μL /Test of Human PD-L1 Protein, Mouse IgG1 Fc Tag (Cat. No. PD1-H52A3) at increasing concentration coupled to Anti-Mouse IgG-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A003) (10 μg beads/Test). Detection was performed with sensitivity of 7.8 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
Protocol
The MPCLIA assay shows that Anti-Mouse IgG-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A003) is stable at 37°C for 10 days.
The MPCLIA assay shows that Anti-Mouse IgG-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A003) is stable after freezing and thawing 3 times.
The MPCLIA assay shows that Anti-Mouse IgG-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A003) is stable at 37°C for 10 days.
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