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- ItemsDetailsDetection MethodChemiluminescenceProduct TypeMagnetic Beads (Anti-His Tag Antibody)Quantity Size10mg / 100mgPhysical Appearancelyophilized powder mixtureParticle size2.8µmBeads SurfaceHydrophilicAmount of Coupled ProteinAbout 133 pmol (20 μg) Monoclonal Mouse Anti-His Tag antibodies/ mg BeadsBinding Capacity3-8μg His tag proteins / mg beadsEmission WavelengthMeasured relative light units (RLU) at 430 nm
背景（Background）
The Anti-His Tag-coupled Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly affinity Monoclonal Mouse Anti-His Tag antibody. The beads can be used to capture the proteins containing N-terminal or C-terminal His Tags (6×His-10×His) in Chemiluminescence procedures. The Monoclonal Mouse Anti-His Tag antibodies are produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with His Tag Protein. The Anti-His Tag-coupled Magnetic Beads is easy to capture his Tagged protein or other molecules, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.
应用说明（Application）
The Anti-His Tag-coupled Magnetic Beads is used to capture his Tagged protein or molecules, it can combination with Acridinium ester markers in chemiluminescence technology, The Acridinium ester markers such as Streptavidin-Acridinium ester can capture the biotinylated proteins or molecules, AHG-Acridinium ester can capture human antibodies, this allows detection of ligand / acceptor binding assay, antigen/antibody binding assay, or antibody screening.
* The Isotype control (Cat. No. SMB-B01) is sold separately.
重构方法（Reconstitution）
See Certificate of Analysis (CoA) for detailed instruction.
存储（Storage）
Upon receipt, please store the Beads at -20℃. The shelf life is 1 year at -20 ℃.
Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.
制剂（Formulation）
Please contact us for detailed information.
Contact us for customized product form or formulation.
质量管理控制体系（QMS）
产品展示
活性（Bioactivity）-MPCLIA
Immobilized 0.025 μg /Test of SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) to the Anti-His Tag-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A005) (10 μg beads/Test), incubated with 100 μL /Test of Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM122) (Cat. No. S1N-M12A1) at increasing concentration coupled to Anti-Human IgG-Acridine ester (Cat. No. AHG-Y69, 0.04 μg /Test). Detection was performed with sensitivity of 0.98 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
Protocol
The MPCLIA assay shows that Anti-His Tag-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A005) is stable at 37°C for 10 days.
The MPCLIA assay shows that Anti-His Tag-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A005) is stable after freezing and thawing 3 times.
The MPCLIA assay shows that Anti-His Tag-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A005) is stable at 37°C for 10 days.
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