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HRP conjugated Monoclonal Anti-SN38 Antibody, Mouse IgG1

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  • 抗体来源(Source)

    HRP conjugated Monoclonal Anti-SN38 Antibody, Mouse IgG1 is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.

  • 种属(Species)

    Mouse

  • 亚型(Isotype)

    Mouse IgG1 | Mouse kappa

  • 偶联(Conjugate)

    HRP-Conjugated

  • 抗体类型(Antibody Type)

    Recombinant Monoclonal

  • 种属反应性(Reactivity)

    Chemical

  • 免疫原(Immunogen)

    SN38-OVA.

  • 特异性(Specificity)

    Specifically recognizes the target-SN38.

  • 应用(Application)

    Application
    Recommended Usage
    ELISA
    2-125 ng/mL 
  • 纯度(Purity)

    90% as determined by SDS-PAGE.

  • 纯化(Purification)

    Protein A purified / Protein G purified

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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  • 电泳(SDS-PAGE)

    SN38 SDS-PAGE

    HRP conjugated Monoclonal Anti-SN38 Antibody, Mouse IgG1 on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • 活性(Bioactivity)-ELISA

     SN38 ELISA

    Immobilized ADC-SN38 at 1 μg/mL (100 μL/well) can bind HRP conjugated Monoclonal Anti-SN38 Antibody, Mouse IgG1 (Cat. No. SN8-PLM685) with a linear range of 2-31.25 ng/mL (QC tested).

    Protocol

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背景介绍

The 7-ethyl-10-hydroxycamptothecin (SN-38) is a topoisomerase I inhibitor (chemotherapeutic agent) used in cancer therapy. In a comparative study of SN-38 with a potent chemotherapeutic prodrug, irinotecan (CPT-11), SN-38 was found to be 1000-fold more cytotoxic against colorectal cancer cells as compared to irinotecan.

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