PE-Labeled Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016) (0.03% Proclin)

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G4S-PFMY25-25tests
$280.00
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G4S-PFMY25-200tests
$1,540.00
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G4S-PFMY25-1000tests (200tests X 5)
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In Stock - Delivery in 2-3 business days
G4S-PFMY25
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Source

Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016) is a rabbit monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which provides higher batch consistency and long term security of supply. It shows superior performance in flow cytometry assays with higher affinity and better specificity.

Antibody Type

Recombinant Monoclonal

Clone

016

Isotype

Rabbit IgG, Kappa

Host Species

Rabbit

Reactivity

All

Conjugate

PE

Excitation Wavelength: 488 nm / 561 nm

Emission Wavelength: 575 nm

Specificity

It can specifically recognize proteins or ScFv containing (G3S)n or (G4S)n linkers, where n = 2, 3, 4... That is, it can recognize linkers with a minimum length of 2 repeats for both (G3S)n and (G4S)n.

Application

Flow Cytometry (Evaluation of cell surface expressed CARs of varying specificity containing a G4S linker within the scFv of the extracellular domain).

Recommended Dilution

1:50

Form

Lyophilized

Formulation

Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300 and 0.2% BSA, with trehalose as protectant.

Please contact us for customized product forms or formulations.

Reconstitution

Please refer to the Certificate of Analysis (CoA) for specific instructions.

Shipping

Lyophilized product is shipped at ambient temperature.

Storage

For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 24 months in lyophilized state;
  • -70°C for 12 months after reconstitution.
  • 2-8°C for 12 months after reconstitution.
Notices

Product Specific Notices: For research use only.

バックグランド

The G4S linker is emerged as the most common linking peptides for scFvs. It is composed of the repeated sequence (Gly-Gly-Gly-Gly-Ser) and commonly found as either a 15-mer (G4S)3 or 20-mer(G4S)4 within scFv fragments. The G4S linker has become an integral part of the vast majority of CARs as it links the VH and VL recognition domains of scFvs. The G4S linker is commonly used in CARs targeting solid tumor antigens, such as HER2 and CEA, while the whitlow/218 linker peptide is predominantly used in CAR constructs targeting hematological malignancies such as CD19 CAR products.