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Monoclonal Anti-Doxorubicin specific Antibody, Rabbit IgG (1M2C3) (MALS verified)

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  • 抗体来源（Source）

    Monoclonal Anti-Doxorubicin specific Antibody, Rabbit IgG (1M2C3) is a Rabbit monoclonal antibody recombinantly expressed from HEK293 cells.

  • 克隆号（Clone）

    1M2C3

  • 种属（Species）

    Rabbit

  • 亚型（Isotype）

    Rabbit IgG | Rabbit Kappa

  • 偶联（Conjugate）

    Unconjugated

  • 抗体类型（Antibody Type）

    Recombinant Monoclonal

  • 免疫原（Immunogen）

    Doxorubicin-BSA.

  • 特异性（Specificity）

    Specifically recognizes Doxorubicin.

  • 应用（Application）

    Application
    Recommended Usage
    ELISA
    0.06-125 ng/mL

  • 纯度（Purity）

    0.95 as determined by SDS-PAGE.

    90% as determined by SEC-MALS.

  • 纯化（Purification）

    Protein A purified / Protein G purified

  • 制剂（Formulation）

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法（Reconstitution）

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储（Storage）

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

  • 交叉验证（Cross Verification）

    This product can cross in Elisa with

    Doxorubicin.

    This product No cross-reactivity in ELISA with

    (PBD)SG3199.

    MMAF.

    Monomethyl auristatin E.

    Trastuzumab Biosimilar.

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

产品展示

  • 电泳（SDS-PAGE）

    Doxorubicin SDS-PAGE

    Monoclonal Anti-Doxorubicin specific Antibody, Rabbit IgG (1M2C3) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 0.95 (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Doxorubicin SEC-MALS

    The purity of Monoclonal Anti-Doxorubicin specific Antibody, Rabbit IgG (1M2C3) (Cat. No. DON-MY2216) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.

    Report

  • 活性（Bioactivity）-ELISA

    Doxorubicin ELISA

    Immobilized Doxorubicin-ADC at 0.1 μg/mL (100 μL/well) can bind Monoclonal Anti-Doxorubicin specific Antibody, Rabbit IgG (1M2C3) (Cat. No. DON-MY2216) with a linear range of 0.06-8 ng/mL (QC tested).

    Protocol

  • Doxorubicin ELISA

    Serial dilutions of Doxorubicin were added into Monoclonal Anti-Doxorubicin specific Antibody, Rabbit IgG (1M2C3) (Cat. No. DON-MY2216): Doxorubicin-ADC binding reactions. The half maximal inhibitory concentration (IC50) is 0.009186 μg/mL (Routinely tested).

    Protocol

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背景介绍

Doxorubicin, a cytotoxic anthracycline antibiotic, is an anti-cancer chemotherapy agent. As the payload of ADC drugs, Doxorubicin can act directly on DNA, insert into the double helix strands of DNA, cause the latter to unwind, change the template properties of DNA, and inhibit DNA polymerase, thereby inhibiting the synthesis of both DNA and RNA. Anti-Doxorubicin antibody is a rabbit monoclonal antibody specifically reacts with Doxorubicin without other payloads, which is more sensitive than mouse antibody. The anti-Doxorubicin antibody is a useful reagent in PK assay to determine conjugated antibodies.

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